METHODS FOR PURIFYING HETERODIMERIC MULTISPECIFIC ANTIBODIES FROM PARENTAL HOMODIMERIC ANTIBODY SPECIES

摘要

To provide methods for purifying multispecific antibodies on interest (MAIs) that co-engage at least two different antigens or epitopes (also referred to targets, used interchangeably throughout), from compositions comprising the MAI and parental homodimeric antibody species.SOLUTION: Also provided are reagents which may be used to practice such methods. This invention particularly provides a method of purifying a multispecific antibody of interest (MAI), wherein the MAI comprises a heterodimer comprising a first polypeptide comprising a first heavy chain (HC) variable region and a second polypeptide comprising a second HC variable region, and advantageously co-engages at least two different antigens or epitopes, from compositions comprising the MAI and at least two corresponding parental homodimeric antibody species.SELECTED DRAWING: None