- Material injection and buffer exchange in microwell arrays with hydrophobic-hydrophilic surfaces

摘要

The present invention relates to a method for injecting a material into a microwell array having a hydrophobic-hydrophilic surface, and the present invention relates to a chamber for culturing microorganisms formed by combining a base and a partition wall, the partition wall being formed and connected to the chamber, A plurality of microwells including an injection hole having a cross-sectional area smaller than the cross-sectional area of the chamber and hydrophobicity respectively formed on the exposed surface of the partition wall where the injection hole is formed and the inner circumferential surface of the injection holes so that the outside of the microwell is more hydrophobic than the inside preparing a microwell array including a material layer; loading and partitioning the microbial suspension into the microwell array; applying a substance for drug screening to the inlet, leaving the substance for drug screening on the inner circumferential surface of the inlet; and raising the humidity around the microwell array to raise the level of the microbial suspension accommodated in the microwell to the height of the inlet, so that the substance for drug screening is mixed with the microbial suspension accommodated in the microwell and injected. A method for injecting a material into a microwell array having a hydrophobic-hydrophilic surface is provided. According to the present invention, when injecting a material into a microwell having a narrow injection hole, a material for drug screening is selectively applied to the injection holes of the microwell using the structure of the injection hole and the hydrophobicity of the injection hole, and water vapor is generated to generate water vapor Just by raising the level of the suspension, a substance for drug screening can be easily injected into a microwell having a narrow injection port, and manual control can be enabled without cross-contamination in the injection process.