Light sheet microscope and method for imaging a sample by light sheet microscopy

摘要

A light sheet microscope includes an illumination optical system configured to generate a light sheet in an intermediate image space. A transport optical system, which is telecentric on both sides, is configured to image the light sheet generated in the intermediate image space into a sample and to image a region of the sample illuminated by the light sheet as an intermediate image into the intermediate image space. A detection optical system is configured to image the intermediate image generated in the intermediate image space onto a detector. The optical axes of the illumination optical system, the transport optical system and the detection optical system intersect one another in the intermediate image space. The microscope further includes a scanning element, which is arranged in the transport optical system and through which the light sheet is moveable in the sample transversely to the optical axis of the transport optical system.