Cucurbit Aphid-Borne Yellows Virus Infectious Clone And Their Uses

摘要

The present invention relates to a Cucumber aphid-borne sulfur virus (CABYV) infectious clone and uses thereof, and more particularly, Agrobacterium tumefaciens ( Agrobacterium tumefaciens ) was used to reproduce the infective activity of the aphid-mediated sulfite virus. As a result of intensive research to reproduce the constant infective activity of Cucurbita Aphid-mediated sulfide virus (CABYV) in Cucurbitaceae plants, the present inventors have found that Agrobacterium transformed using a recombinant plasmid containing the Cucurbita aphid-mediated sulfide virus gene. It was confirmed for the first time that this Cucurbitaceous plant can infect Cucurbita and aphid-mediated sulfide virus, Agrobacterium tumefaciens according to the present invention is a constant infection of Cucurbita aphid-mediated sulfide virus (CABYV). By reproducing the activity in the Cucurbitaceae plant, it will be applied to study the function of the protein expressed by the virus and to elucidate pathogenic factors, and it is expected to be usefully utilized in research for the cultivation of Cucumber aphid-mediated sulfide virus (CABYV) resistant varieties. It is expected.