TIBETAN PIG FECAL MICROBIOTA CAPSULE FOR RELIEVING DIARRHEA IN WEANED PIGLET, AND PREPARATION METHOD THEREFOR

摘要

The present application belongs to the technical field of veterinary medicine, and discloses a method for preparing a Tibetan pig fecal microbiota capsule for relieving diarrhea in a weaned piglet. The method comprises collecting fresh rectal fecal microbiota from a Tibetan pig and preparing a Tibetan pig fecal microbiota suspension; centrifuging and purifying the Tibetan pig fecal microbiota suspension, removing supernatant, and mixing the supernatant with a sodium alginate solution, whey protein isolate, sucrose, mannitol and glycerol evenly according to a ratio; preparing the mixed solution into Tibetan pig fecal microbiota gel particles, allowing the gel particles to stand, and then further packaging the gel particles in a methylcellulose capsule shell to obtain the Tibetan pig fecal microbiota capsule. The Tibetan pig fecal microbiota capsules prepared by the present method completely preserve the bacterial diversity and activity in the Tibetan pig fecal microbiota. Moreover, the added glycerol, among others, can increase the activity of the bacteria in the Tibetan pig fecal microbiota capsule, enhance the effect of administering of an intestinal flora preparation, and significantly decrease the mortality rate of weaned piglets due to diarrhea.