YAP1 GENE-MODIFIED MESENCHYMAL STEM CELL AND PREPARATION METHOD THEREOF

摘要

Provided is a YAP1 gene-modified mesenchymal stem cell and a preparation method thereof. The mesenchymal stem cell is a primary mesenchymal stem cell modified by overexpressing a YAP1 gene. The YAP1 gene is derived from a YAP1 lentiviral vector or a YAP1 plasmid vector. The primary mesenchymal stem cell is derived from any one of human placental, umbilical cord or adipose tissue. The resultant YAP1 gene-modified mesenchymal stem cell does not affect the phenotype and differentiation ability of the MSC itself. By modifying the mesenchymal stem cell through the overexpression of the YAP1 gene, proliferation of the mesenchymal stem cell can be significantly promoted and the cell yield further increased, thereby quickly obtaining a large number of mesenchymal stem cells for clinical stem cell transplantation treatment.