LABEL-FREE NANOSENSORS FOR DETECTION OF GLYCOPROTEINS

摘要

A method for detecting glycoproteins in aqueous samples. The method includes putting an aqueous sample in contact with a diagnostic kit, obtaining an electrochemical pattern of the aqueous sample by applying an electrical potential to the diagnostic kit, and detecting a glycoprotein status of the aqueous sample based on the presence of a peak in the electrochemical pattern of the aqueous sample. The diagnostic kit includes a counter electrode, a reference electrode, and a working electrode including a label-free nanosensor deposited on a substrate. The label-free nanosensor includes a modified graphene oxide (GO) sheet and a signal amplifying agent loaded onto the modified GO sheet. The modified GO sheet includes a modifying agent conjugated to a GO sheet. The modifying agent includes 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC), N-hydroxysuccinimide (NHS), 8-hydroxyquinoline (8H), and hydroxylammonium chloride. The signal amplifying agent includes at least one of an amine-functionalized gold nanoparticle and a silver nanoparticle.