Differentiation induction method to germ cell lines using undifferentiated germ cells with enriched living ability

摘要

To provide a method for inducing differentiation of isolated germ cells into a germ line that is adapted for practical use by improving the engraftment ability of the transplanted germ cells into the host gonads and increasing the transplant efficiency in the surrogate parent fish farming method To the germline of isolated germ cells consisting of transplanting the isolated germ cells derived from fish isolated from the testis or ovary of the fish into the host fish individual by intraperitoneal transplantation of the host fish before and after hatching In the method of inducing differentiation, an antibody specifically recognizing an undifferentiated germ cell capable of engrafting in the host gonad is obtained in advance, and the antibody is used to transplant the host fish into the peritoneal cavity. By separating and concentrating spermatogonia that have the ability to engraft the host gonads from the isolated germ cells, and transplanting the separated germ cells into the peritoneal cavity of the host fish before and after hatching, the germ cells into the host fish gonads of Improve Chakuno by providing a method for inducing differentiation into germ line separating germ cells, to solve the problem.