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METHOD FOR PREPARING PRECURSOR OF RECOMBINANT HUMAN INSULIN OR ANALOGUE THEREOF
METHOD FOR PREPARING PRECURSOR OF RECOMBINANT HUMAN INSULIN OR ANALOGUE THEREOF
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机译:制备重组人胰岛素或其类似物的前体
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摘要
Disclosed is a method for preparing a precursor of a recombinant human insulin or an analogue thereof, comprising: a. bacterial fermentation, centrifuging a fermentation broth in a continuous flow to collect a supernatant; b. filtering the supernatant in step (a) by means of a hollow fiber membrane and collecting the filtrate; and c. purifying the filtrate in the step (b) by means of a chromatography column. The method has the advantages of streamlined steps, scalability, no use of organic solvents, high yield, etc. The purity of the insulin precursor can exceed 90%, the host cell protein removal rate exceeds 90%, and the exogenous DNA removal rate is 89% or greater, achieving less than 0.1 ng/mg.
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机译:公开了一种制备重组人胰岛素或其类似物的前体的方法,包括:a。细菌发酵,在连续流动中离心发酵液以收集上清液;湾通过中空纤维膜在步骤(a)中过滤上清液并收集滤液;和c。通过色谱柱纯化步骤(b)中的滤液。该方法具有精简的步骤,可扩展性,无种油溶剂,高产率等的优点。胰岛素前体的纯度可以超过90%,宿主细胞蛋白去除率超过90%,外源性DNA去除率是89%或更高,达到少于0.1 ng / mg。
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