HIGH-THROUGHPUT NUCLEOTIDE LIBRARY SEQUENCING

摘要

Provided herein are methods and composition for immune repertoire sequencing and single cell barcoding. The methods and compositions can be used to pair any two sequences originating from a single cell, such as heavy and light chain antibody sequences, alpha and beta chain T-cell receptor sequences, or gamma and delta chain T-cell receptor sequences,for antibody and T-cell receptor discovery, disease and immune diagnostics, and low error sequencing.