NON–INVASIVE AND LABEL–FREE METHOD FOR EVALUATION OF BIOCHEMICAL STATE OF PACKED RED BLOOD CELLS

摘要

A non–invasive and label–free method for evaluating the quality of packed red blood cells (pRBCs), where the pRBCs is largely red blood cells (RBCs) and a supernatant mixture (SM), along with trace amounts of other blood cell components including leukocytes and blood plasma. The measurement comprises at least one Raman spectrum of the pRBCs and a subsequent chemometric analysis. In such a way, at least one Raman spectrum is collected from the SM and a chemometric analysis of the collected spectrum is carried out for at least one ratio of the integral intensities of the chosen Raman marker bands within this spectrum. Subsequently, the result that is obtained for at least one ratio of the integral intensities is compared with a defined reference value, that is, either the reference value of the pRBCs' expiration (RV-Exp) or restricted reference value (RV-Res) of the pRBCs' quality, to assess the utility of the blood for transfusion to a chosen group of patients.