GUIDE RNA FOR CONDITIONAL AND POST-TRANSCRIPTIONAL CONTROL OF CRISPR-CAS PROTEIN

摘要

The present invention relates to a CRISPR-Cas system guide RNA consisting of one or more polynucleotide(s), wherein the guide RNA comprises: (A) the following elements (a) to (d), arranged in this order from 5' to 3': (a) a spacer which can hybridize to a target polynucleotide; (b) a first functional module comprising a crRNA:tracrRNA duplex; (c) a second functional module comprising a first switching domain; and optionally (d) a third functional module; and (B) a second switching domain, wherein at least a portion of the first switching domain is substantially complementary to at least a portion of the second switching domain, and when an opener is present, the opener disrupts the hybridization between the first switching domain and the second switching domain and the guide RNA forms at least one stem within the first switching domain, and thereby becomes capable of activating a Cas protein. The present invention provides conditional activation of CRISPR-Cas system using various kinds of triggers, for example an endogenous molecule.