Method for detection of target point mutations based on polymerase chain reaction and DNA ligation reaction

摘要

The present invention relates to a method for detecting a target point mutation of a base sequence based on a polymerase chain reaction (PCR) and a DNA ligation reaction, and specifically, when a target point mutation is present, a target point mutation and a template A cyclic template is formed to perform an amplification reaction, but the product generated during the amplification reaction induces an additional amplification reaction, thereby detecting a target point mutation having an effect of increasing the reaction rate and sensitivity, and a polymerase chain reaction for implementing the method ( Polymerase Chain Reaction (PCR)), and the target point variation analysis method according to the present invention not only can simultaneously analyze multiple targets in a single reaction using a template prepared according to the type of each target point variation, Since it is possible to analyze the displacement of the base unit with high sensitivity, it is useful for the analysis of point variations. In addition, since the length of the product is constant, no additional cleavage enzyme is required, and if the length of the product binding to the probe barcode is too long when analyzing on the sensor surface, the analysis will not be accurate, but the product of the invention has a certain length. Since various existing gene analysis systems can be used, it is useful for detecting gene mutations.