Enhancement of RNA directed genome editing specificity using RNA derived fkii nuclease (RFN)

摘要

To provide methods for increasing specificity of RNA-guided genome editing, e.g., editing using CRISPR/Cas9 systems.SOLUTION: The invention provides, among others, FokI-dCas9 fusion proteins, comprising a FokI catalytic domain sequence fused to the terminus, e.g., the N terminus, of dCas9, optionally with an intervening linker, e.g., a linker of 2-30 amino acids, e.g., 4-12 amino acids, e.g., Gly4Ser.SELECTED DRAWING: Figure 5A