PROBLEM TO BE SOLVED: To provide a method for examining ulcerative colitis, which is useful for determining a disease state of ulcerative colitis. SOLUTION: This is a method for producing a bacterial species sample derived from a subject in which the bacterial species diversity and / or the number of bacterial species in the sample of the subject is maintained, and 1) the sample of the subject is added to a medium. Steps, 2) a step of performing an operation so that the pH of the medium becomes 6.2 to 6.7 as necessary at the start of culturing, and 3) maintaining the pH of the medium after the start of culturing. The method includes a step of culturing the sample without performing an intended operation. Preferably, prior to 1), A) the step of sterilizing the medium and aeration to an anaerobic gas and / or B) the step of suspending the sample in an ascorbic acid-added phosphate buffer are included. .. [Selection diagram] Fig. 1
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