Screening method for genes related to self-renewal ability in hematopoietic stem cells and usage of the genes

摘要

【Task】 A method for separating long-term hematopoietic stem cells and short-term hematopoietic stem cells, a method for identifying genes specifically expressed in long-term hematopoietic stem cells, a method for identifying genes involved in long-term self-renewal ability maintenance like long-term hematopoietic stem cells, and the method thereof. To provide a method for imparting or enhancing long-term self-renewal ability maintenance characteristics in hematopoietic stem cells using such genes. SOLUTION: By using the Hoxb5 gene as a marker as a gene expressed only in long-term hematopoietic stem cells, it is possible to separate long-term hematopoietic stem cells and short-term hematopoietic stem cells. It has become possible to screen the genes involved. In fact, it was confirmed that the self-renewal ability maintenance property is enhanced by expressing the Hoxb5 gene specifically expressed in long-term hematopoietic stem cells in short-term hematopoietic stem cells. [Selection diagram] None