The present invention relates to a CRISPR/Cas system comprising guide RNAs and a Cas protein, wherein the guide RNAs are composed of the following two parts: the guide RNAs are capable of respectively binding to two sequences of target sequences having SEQ ID NO: 2, SEQ NO: 3 and SEQ ID NO: 4 in eukaryotic B2M genes. The system exhibits a relatively high single-gene knockout rate for B2M. The present invention further relates to a gene editing method for T cells using the system, which method provides an efficient, safe and simple process for producing universal T cells.
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机译:本发明涉及一种包括引导RNA和CAS蛋白的CRISPR / CAS系统,其中引导RNA由以下两部分组成:引导RNA能够分别结合具有SEQ ID NO:2的靶序列的两种序列。 ,SEQ NO:3和SEQ ID NO:4在真核B2M基因中。该系统表现出B2M的相对高的单基因敲除率。本发明还涉及使用该系统的T细胞的基因编辑方法,该方法提供了用于产生通用T细胞的有效,安全和简单的方法。
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