METHOD FOR MEASURING ACTIVITY OF LIPID-METABOLIZING ENZYME IN CELL, AND SUBSTANCE FOR USE IN MEASUREMENT OF ACTIVITY OF LIPID-METABOLIZING ENZYME IN CELL

摘要

Some types of DGK isozymes have been difficult to measure the activities thereof in cells.　A method for measuring the activity of a diacylglycerol kinase comprises the steps of: introducing a diacylglycerol kinase having a myristoylation sequence into a cell; and measuring the amount of phosphatidic acid in the cell. The diacylglycerol kinase having a myristoylation sequence is at least one nucleotide sequence selected from the nucleotide sequences (1) to (3). (1) A nucleotide sequence represented by SEQ ID NO: 1; (2) a nucleotide sequence having 90% or more identity to the nucleotide sequence represented by SEQ ID NO: 1; and (3) a nucleotide sequence having such a structure that 5 or less nucleotides in a nucleotide sequence comprising the nucleotide sequence represented by SEQ ID NO: 1 are deleted, substituted and/or added.