Enzyme activity measurement method , enzyme activity measuring device and enzyme activity measurement program

摘要

PROBLEM TO BE SOLVED: To accurately measure an enzyme activity in a short time.;SOLUTION: The measuring area of a target body 4 is irradiated with optical quenching light to quench HFC (hydrofluorocarbon), and a point of time when quenched is set to be a start time T0 for recovery. The intensity of fluorescence emitted from a newly metabolized HFC is measured as a fluorescence value. By calculating the enzyme activity based on the fluorescence value and the measurement time, the fluorescence value at the recovery start time T0 is reduced and further, the percentage of the fluorescence value varying with the elapse of time with respect to the recovery start time T0 can be enlarged, thus measuring the enzyme activity accurately in a short time.;COPYRIGHT: (C)2011,JPO&INPIT