Non-toxic HSV vectors for efficient gene delivery applications and complementing cells for their production

摘要

In one embodiment, the present invention provides a herpes simplex virus (HSV) vector that does not express a toxic HSV gene in a non-complementary cell, wherein the vector is capable of expressing a transgene for at least 28 days in a non-complementary cell. have. The invention also relates to stocks of the vectors of the invention, their compositions suitable for therapeutic use or in vitro application, and methods relating to them. In another aspect, the present invention provides a complementary cell engineered to express ICP4 and ICP27 when the cell is infected with HSV for production of the vector of the present invention.