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METHODS AND KITS FOR DETECTING O-GlcNAc SITES USING B3GALNT2 AND OGT
METHODS AND KITS FOR DETECTING O-GlcNAc SITES USING B3GALNT2 AND OGT
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机译:使用B3GalnT2和OGT检测O-GlcNAC位点的方法和套件
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摘要
In an in vitro method of determining the modification degree of O-GlcNAc in a biological sample, a first sample is treated with B3GALNT2 to incorporate a GalNAz into closed O-GlcNAc sites in the sample, labeled using click chemistry to form labeled closed O-GlcNAc sites, and the labeled closed O-GlcNAc sites in the first sample are detected (corresponding to the number of closed O-GlcNAc sites in the biological sample). A second, duplicate sample is treated with OGT to O-GlcNAcylate open O-GlcNAc sites in the sample to convert the open O-GlcNAc sites to closed O-GlcNAc sites, and treated with B3GALNT2 to incorporate GalNAz into the closed O-GlcNAc sites. The second sample is labeled using click chemistry to form labeled closed O-GlcNAc sites (corresponding to the total number of O-GlcNAc sites in the biological sample). The number of closed O-GlcNAc sites and total O-GlcNAc sites in the biological sample are compared to determine the modification degree of O-GlcNAc.
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