Differentiation of human embryonic stem cells into single hormone insulin-positive cells

摘要

PROBLEM TO BE SOLVED: To provide a method for promoting the differentiation of pluripotent stem cells, specifically, a method for generating a cell population, in which more than 10% of cells are characteristic of pancreatic β cells of a single hormone. Marker is expressed. An in vitro differentiated population of pancreatic endoderm cells obtained from the stepwise differentiation of pluripotent cells, in which the cells at each stage of differentiation are cultured in a medium containing 5 mM to 20 mM glucose. Differentiated population of pancreatic endoderm cells. Over 30% of the differentiated pancreatic endoderm cells are PDX-1 + NKX6.1 +, SOX2-, and CDX2-. [Selection diagram] None