Antibacterial phage, therapeutic composition, bactericidal agent, food, bacterial discrimination kit, therapeutic composition manufacturing method, bacterial removal method, bacterial discrimination method, and animal therapeutic method

摘要

Provided are antibacterial phages that selectively kill bacteria having a drug resistance gene or the like. Antibacterial phage for this include CRISPR-Cas13a with a target sequence that recognizes a specific gene as a target. This target sequence is designed as a spacer sequence for crRNA of 14-28 bases. Specific genes are drug resistance genes and toxins. Drug resistance genes include methicillin-resistant yellow staphylococcus, bancomycin-resistant yellow staphylococcus, bancomycin-resistant enterococcus, penicillin-resistant pneumococcus, multidrug-resistant pyogenes, multidrug-resistant Asinetobacter, carbapenem-resistant seracia, It is contained in the genome and / or plasmid of a bacterium containing one or any combination of the group consisting of third-generation cephalosporin-resistant pneumoniae, third-generation cephalosporin-resistant E. coli, and fluoroquinolone-resistant E. coli.