Manufacture of manool

摘要

Contacting geranylgeranyl diphosphate with coparyl diphosphate (CPP) synthase to form (9S, 10S) -coparyl diphosphate and contacting CPP with a sclareol synthase enzyme (+)-manol Wherein CPP synthase is at least 90%, 95%, 98 relative to a polypeptide selected from the group consisting of SEQ ID NO: 1 and SEQ ID NO: 2. Said method is provided herein comprising amino acid sequences exhibiting%, 99% and 100% sequence identity.