A patterned optoelectronic tweezers (p-OET) device is provided. The p-OET device includes a top and a bottom electrode arranged in a parallel spaced apart relationship. A patterned photoconductor layer is provided on the bottom electrode, and forms a pattern comprising at least one raised region where the bottom electrode is coated by a photoconductor material and at least one hollow region where the bottom electrode is exposed. The pattern includes one or more boundaries between the raised and hollow regions. In some implementations, the boundaries of the patterned photoconductive layer define a permanent trap feature.
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