Water-soluble fluorescent probe and nanoparticles with agglutination-induced emission effect for use in ovarian cancer, and their manufacturing method and application

摘要

The present invention discloses a water-soluble fluorescent probe having an agglutination-induced emission (AIE) effect, nanoparticles, and manufacturing methods and applications. The fluorescent probe molecule contains two methoxyphenyl and phenyl-substituted imidazole rings and two quaternary ammonium salt structures, and exhibits excellent dispersibility in water and can form micelles. In addition, it is suitable for fluorescence detection because it has the characteristics of agglutination-induced emission effect and stroke shift. Under the induction of lysophosphatidic acid, since the probe can be self-assembled, it can aggregate to generate yellow fluorescence, and quantitative analysis of lysophosphatidic acid can be implemented by measuring the intensity of the emitted light. This has advantages such as excellent specificity, high sensitivity, and resistance to interference. It is particularly suitable for chemical solution systems, and the detection of lysophosphatidic acid in an environment such as a biological sample is very suitable for the detection of trace amounts of lysophosphatidic acid, detection of the content of lysophosphatidic acid in plasma of ovarian cancer patients, and early clinical diagnosis and drug use guidelines. It has an important practical value.