The present invention is a method for cryopreserving sperm containing egg yolk extract and milk protein extract, a method for cryopreserving sperm of cattle using a composition for cryopreservation of sperm containing the egg yolk extract and milk protein extract, and cryopreserved by the above method. Cattle breeding method comprising the step of thawing sperm and then injecting it into a cow during ovulation, and after thawing the frozen sperm by the above method, injecting a fertilized egg formed by in vitro fertilization into a cow during ovulation It's about the method. When using the cryopreservation composition provided in the present invention to cryopreserve sperm of cattle, the survival rate of sperm after thawing is improved, and thus, it will be widely used for effective breeding and breed management of cattle.
展开▼
