The present invention is a composition for cryopreservation of sperm comprising egg yolk extract and milk protein extract, a method for cryopreserving bovine sperm using the composition for cryopreservation of sperm comprising egg yolk extract and milk protein extract, and cryopreserved by the method After thawing the sperm, the method of breeding a cow comprising the step of injecting into the cow of the ovulatory period, and thawing the sperm cryopreserved by the above method, and then injecting the fertilized egg formed by in vitro fertilization into the cow of the ovulatory period. It's about how. When cryopreservation of sperm in a cow using the composition for cryopreservation provided in the present invention improves the survival rate of sperm after thawing, it may be widely used for effective breeding and breed management of cows.
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