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EXPRESSION OF KLEBSIELLA OXYTOCA POLYPEPTIDES INVOLVED IN LYSINE DECARBOXYLATION, AND METHODS AND APPLICATIONS THEREOF
EXPRESSION OF KLEBSIELLA OXYTOCA POLYPEPTIDES INVOLVED IN LYSINE DECARBOXYLATION, AND METHODS AND APPLICATIONS THEREOF
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机译:Klebsiella ocktoca多肽参与赖氨酸脱羧的多肽,以及其方法和应用
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摘要
One aspect provided herein relates to lysine decarboxylase polypeptides comprising mutants of SEQ ID NO: 2 (i.e., mutants of Klebsiella oxytoca (K. oxytoca) Ldc) and/or fragments thereof. In certain embodiments, the mutants or fragments thereof may have at least about 95% sequence identity with SEQ ID NO: 2. Another aspect provided herein relates to a DNA polynucleotide comprising one or more lysine decarboxylase nucleotide sequences of mutants of SEQ ID NO: 1 (i.e., mutants of K. oxytoca ldc), fragments thereof, or fragments of SEQ ID NO: 1 (i.e., fragments of K. oxytoca ldc). In certain embodiments, the DNA polynucleotide as disclosed herein may encode one or more lysine decarboxylase polypeptides provided herein. In certain embodiments, the DNA polynucleotide as disclosed herein may encode SEQ ID NO: 2, mutants, and/or fragments thereof. In certain embodiments, the lysine decarboxylase nucleotide sequences provided herein may have at least 95% sequence identity with SEQ ID NO: 1 or SEQ ID NO: 3. Another aspect provided herein relates to expression vectors comprising the DNA polynucleotides described herein used for production of a lysine-derived product. Other aspects provided herein include transformants, mutant host cells, methods for the production of lysine decarboxylases, and methods for the production of a lysine-derived product.
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机译:本文提供的一个方面涉及包含SEQ ID NO:2的突变体的赖氨酸脱羧酶多肽(即,Klebsiella催腺素催腺素的突变体(K oxytoca)和/或其片段。在某些实施方案中,其突变体或其片段可以具有至少约95%的序列同一性,其具有SEQ ID NO:2。本文提供的另一方面涉及包含SEQ ID NO:1的突变体的一种或多种赖氨酸脱羧酶核苷酸序列的DNA多核苷酸。 (即,K. oxytoca Ldc的突变体),其片段或SEQ ID NO:1的片段(即K. oxytoCa LDC的片段)。在某些实施方案中,如本文所公开的DNA多核苷酸可以编码本文提供的一种或多种赖氨酸脱羧酶多肽。在某些实施方案中,如本文所公开的DNA多核苷酸可以编码SEQ ID NO:2,突变体和/或其片段。在某些实施方案中,本文提供的赖氨酸脱羧酶核苷酸序列可具有至少95%的序列同一性,具有SEQ ID NO:1或SEQ ID NO:3。本文提供的另一方面涉及包含本文所述的DNA多核苷酸用于生产的DNA多核苷酸的表达载体赖氨酸衍生的产品。本文提供的其他方面包括转化体,突变宿主细胞,制备赖氨酸脱羧酶的方法,以及制备赖氨酸衍生产物的方法。
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