Method of enzymatic hydrolysis of the materials protein and of autolysis of the animal tissues eliminating the process of microbial development.

• 1. 壳多糖、水解产物和借助于酶促水解的方式用于从昆虫生产至少一种目标期望的产物的方法，其包括在酶促水解前执行的步骤组合 [P] . 中国专利： CN107278208A . 2017-10-20
• 2. 微生物菌种W，含该微生物的酶组合物，该微生物的应用及尤其水解角蛋白的方法 [P] . 中国专利： CN1082108A . 1994-02-16
• 3. A method for the enzymatic hydrolysis of protein substances and autolysis of animal tissues [P] . 德国专利： DE946061C . 1956-07-26

  机译： 蛋白物质的酶促水解和动物组织自溶的方法

• 4. process for enzymatic hydrolysis of eiweissstoffen and autolysis of animal tissues [P] . 德国专利： DE000000946061B . 1956-07-26

  机译： 威斯托芬的酶解过程及动物组织的自溶过程

• 5. PROCESS FOR PREPARING ANIMAL FEEDS FOR THE SUBSTITUTION OF MILK WITH THE ENZYMATIC HYDROLYSIS OF RAW MATERIALS CONTAINING PROTEIN OF PLANT OR ANIMAL ORIGIN [P] . HU171944B . 1978-04-28

  机译： 含植物或动物蛋白的原料经酶解水解后代乳的动物饲料制备方法

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• 4. The Relationship between the Hydrolysis Degree and Calcium-binding Capacity of Whey Protein by Enzymatic Hydrolysis [C] . Lina Zhao, Shaoyun Wang, Shunli Huang, International Conference on Mechanical, Industrial, and Manufacturing Technologies . 2014
• 5. PARTIAL ACID HYDROLYSIS PRETREATMENT FOR ENZYMATIC HYDROLYSIS OF CELLULOSE: A PROCESS DEVELOPMENT STUDY FOR ETHANOL PRODUCTION. [D] . KNAPPERT, DIANE RUTH. 1981
• 6. Torque measurements reveal large process differences between materials during high solid enzymatic hydrolysis of pretreated lignocellulose [O] . Benny Palmqvist, Gunnar Lidén 2012
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• 1. BACKGROUND： Vibrio vulnifi cus inside the body could activate the NF-!B signaling pathwayand initiate the inflammatory cascade. The lung is one of the earliest organs affected by sepsisassociated with acute lung injury. High mobility group protein B1 （HMGB1） is an important late-actingpro-infl ammatory cytokine involving in the pathophysiology of sepsis. It is also involved in the injuryprocess in the lung, liver and intestine. There has been no report on the involvement of HMGB1 inVibrio vulnifi cus sepsis-induced lung injury.METHODS： Sixty rats were randomly divided into a normal control group （group A, n=10） anda Vibrio vulnificus sepsis group （group B, n=50）. Sepsis was induced in the rats by subcutaneousinjection of Vibrio vulnificus （concentration 6×108 cfu/mL, volume 0.1 mL/100g）） into the left lowerlimbs. The rats in group B were sacrifi ced separately 1, 6, 12, 24, and 48 hours after the infection.Their lungs were stored as specimens, lung water content was measured, and lung pathology wasobserved under a light microscope. The expressions of the HMGB1 gene and protein in the lungswere detected by RT-PCR and Western blot. Data were analyzed with one-way analysis of variance（ANOVA） and the LSD method for pair-wise comparison between the two groups. P〈0.05 wasconsidered statistically signifi cant.RESULTS： Compared to group A （0.652±0.177）, HMGB1 mRNA expression in the lungs ofgroup B was signifi cantly higher at 0 hour （1.161±0.358, P=0.013）, 24 hours （1.679±0.235, P=0.000）,and 48 hours （1.258±0.274, P=0.004） （P〈0.05）, and peaked at 24 hours. Compared to group A（0.594±0.190）, HMGB1 protein expression at 6 hours （1.408±0.567, P=0.026） after infection wassignificantly increased （P〈0. 05）, and peaked at 24 hours （2.415±1.064, P=0.000） after infection.Compared to group A （0.699±0.054）, lung water content was significantly increased at 6 hours（0.759±0.030, P=0.001）,12 hours （0.767±0.023, P=0.000）, 24 hours （0.771±0.043, P=0.000） and 48hours （0.789±0.137, P=0.000） after infection （P〈0.05）. Compared to group A, pathological changesat 12 hours in group B indicate marked pulmonary vascular congestion, interstitial edema andinfl ammatory infi ltration. Alveolar cavity collapse and boundaries of the alveolar septum could not beclearly identifi ed.CONCLUSION： Vibrio vulnifi cus sepsis can lead to injury in rat lungs, and increased HMGB1expression in lung tissue may be one of the mechanisms for injury from Vibrio vulnifi cus sepsis. [J] . Qiao-meng Qiu ,Zhong-wang Li ,Lu-ming Tang . 世界急诊医学杂志(英文) . 2011,第4期
• 2. Antioxidant Activities of Okra Protein Concentrate and Isolate after Enzymatic Hydrolysis [J] . Abdulkareem A. Kareem ,Khalidah A. Shakir ,Marie K. Walsh . 食品与营养科学（英文） . 2018,第9期
• 3. 脱脂菜籽饼粕中蛋白质的分步酶水解研究V.中性蛋白酶与木瓜蛋白酶分步水解 [C] . 熊善柏 ,卢金珍 ,刘海梅 . 第四届中国农学会农产品储藏加工分会学术交流会 . 2001
• 4. 酪蛋白酶促水解过程集总动力学研究 [A] . 于艳军 . 2003