A procedure for the production of a attenuated vaccine to immunize the pigs against the colera. (Machine-translation by Google Translate, not legally binding)

摘要

A method of producing an attenuated vaccine to immunize pigs against cholera, characterized by introducing an inoculum of cholera virus capable of producing a cytopathogenic effect in its original passage through a nontoxic liquid tissue culture medium containing viable cells of pig kidney tissue, incubating said tissue culture medium until a cytopathogenic effect occurs on said tissue and then serially passing the virus through a series of at least 30 pig kidney tissue cultures. (Machine-translation by Google Translate, not legally binding)