A method for preparing an inactivated porcine cholera vaccine, characterized by developing a vaccine virus by infecting a single layer pig tissue culture with porcine cholera active virus, at a ph of about 7.2 to about 7.6, until a substantial repetition of the virus has taken place, cooling said resulting vaccine virus to the freezing state, and then heating said vaccine virus and recovering the active virus; deactivate said active virus; and collect the inactivated vaccine product. (Machine-translation by Google Translate, not legally binding)
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