the catalytic system with a sustainable and increased enzyme activity stability

摘要

1415597 Reactions with absorbed enzymes NATIONAL PATENT DEVELOPMENT CORP 1 Feb 1973 [11 Feb 1972] 5197/73 Heading B1X [Also in Divisions C3 and G1] A process for subjecting a substrate e.g. blood or body fluid containing an enzymesusceptible material to the action of an enzyme comprises permitting the enzyme-susceptible material of the substrate to come into contact with the enzyme entrapped or encapsulated within a water-insoluble hydrophilic polymer of a monomer of the group consisting of hydroxy lower alkyl (i.e. C 1 -C 3 ) acrylates, hydroxy lower alkyl methacrylates, hydroxy lower alkoxy lower alkyl acrylates, hydroxy lower alkoxy lower alkyl methacrylates with or without a proportion of vinyl pyrrolidone, acrylamide, methacrylamide, N- lower alkyl acrylamide, N-lower alkyl methacrylamide, N-hydroxy lower alkyl acrylamide, and N-hydroxy lower alkyl methacrylamide. The water insoluble hydrophilic polymer may be in the form of a hollow tube having a relatively thin overall internal semipermeable membrane coating of a polymer as above, but enzyme free, the semi-permeable membrane being permeable to the enzyme-susceptible material but not to the enzyme. In Fig. 3 a tube 18, shown in crosssection, comprises a lumen 20, a urease containing polymer layer 22 containing a reinforcing sheathing of polyethylene terephthalate and, optionally, inner and outer coatings of water insoluble hydroxyethyl methacrylate polymer. When a solution of urea is forced through the lumen of the tube the solution diffuses to the enzyme entrapped tube and ammonia diffuses out of the outer surface of the tube thus enabling the purification of body fluids e.g. blood to eliminate urea.