procedure for the analytical determination of the nucleotideinheiten in polynucleotiden

摘要

Sequential analysis of a polynucleotide to determine the particular order of nucleoside units therein can be conveniently carried out by adsorbing a polynucleotide on a strongly basic anion-exchange material, oxidizing the terminal nucleoside of the polynucleotide with a periodate, removing any excess periodate by reaction with L-rhamnose, treating the adsorbed polynucleotide with an amine to remove the terminal nucleoside residue from the polynucleotide molecule and with a phosphatase to remove the resulting terminal phosphate group from the remaining polynucleotide molecule, separating the so-produced nucleoside residue from the adsorbed polynucleotide for subsequent identification and then repeating the above procedure for each remaining nucleoside unit of the polynucleotide.