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procedure for the analytical determination of the nucleotideinheiten in polynucleotiden
procedure for the analytical determination of the nucleotideinheiten in polynucleotiden
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机译:分析法测定多核苷酸中的核苷酸的方法
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摘要
Sequential analysis of a polynucleotide to determine the particular order of nucleoside units therein can be conveniently carried out by adsorbing a polynucleotide on a strongly basic anion-exchange material, oxidizing the terminal nucleoside of the polynucleotide with a periodate, removing any excess periodate by reaction with L-rhamnose, treating the adsorbed polynucleotide with an amine to remove the terminal nucleoside residue from the polynucleotide molecule and with a phosphatase to remove the resulting terminal phosphate group from the remaining polynucleotide molecule, separating the so-produced nucleoside residue from the adsorbed polynucleotide for subsequent identification and then repeating the above procedure for each remaining nucleoside unit of the polynucleotide.
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