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procedures for the extraction of virgin mikrobiellem protein with a low nucleinsaeuregehalt, as is nahrungsoder feed

机译:低核素基因型低蛋白的原始mikrobiellem蛋白的提取方法,那伦索德饲料

摘要

Cell material consisting of unicellular microorganisms is ground in the form of a 1-25% suspension with the addition of alkaline substances (pref. NaOH) at pH 9-14 at 0-40 degrees C, the cell walls are separated off at pH 7-9, the protein is precipitated by acidifying at pH 2-7 and the protein is centrifuged off, dried extracted with alcohol to remove lipids, and again dried to give protein having a low nucleic and content which is utilisable as a food- or feedstuff. In the above procedure, nuclease is released during the grinding process so that nucleic acids are broken down to nucleotides which are not precipitated with the protein. The protein obtained is more easily and has a higher biological value than protein contained in intact cells.
机译:由单细胞微生物组成的细胞材料以1-25%悬浮液的形式进行研磨,并在0-40℃下在pH 9-14下添加碱性物质(优选NaOH),在pH 7下分离出细胞壁-9,通过在pH 2-7酸化沉淀蛋白质,将蛋白质离心分离,用酒精干燥以除去脂质,然后再次干燥得到核酸和含量低的蛋白质,可用作食品或饲料。在上述步骤中,核酸酶在研磨过程中释放出来,因此核酸被分解为不会随蛋白质沉淀的核苷酸。与完整细胞中包含的蛋白质相比,获得的蛋白质更容易并且具有更高的生物学价值。

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