a method and a device for monitoring run and identification of particles analyzed by a doorstroominrichting.

摘要

1485284 Characterization of suspended particles COULTER ELECTRONICS Inc 18 Oct 1974 [19 Oct 1973 9 Aug 1974] 45323/74 Heading G1B Apparatus for obtaining information on the characteristics of particles in a liquid suspension comprises a particle scanning device 14 comprising means for containing a quantity of particulate suspension, a sensing zone responding to passage of each particle to produce a signal representing at least one physical characteristic of the particle, means to move the suspension in a stream 27 from the container through the sensing zone and out of the particle scanning device, means for producing signals representing the temporal pattern generated by the passing of the successive particles through the sensing zone with the particles spaced along the stream in a spatial pattern and means for preserving the signals representing the temporal pattern with each signal identified in the pattern with the particle producing it, a substrate 48 arranged to intercept the stream emerging from the scanning device, means for effecting relative movement between the substrate and the stream whereby the stream, which may be continuous or in droplet form, is laid down as a track on the surface of the substrate with the spatial arrangement of the particles preserved on the substrate surface and means 40, 36 for correlating the spatial and temporal patterns to match each of the particles in the track with the signals representing the physical characteristics of the particles. The substrate may be a slide (18), Fig. 1 (not shown), having a surface of frosted or fritted glass to assist in the retention of droplets of suspension, or a pressed paper, or resin optionally in the form of a tape as shown, when the suspension medium is sucked away from the deposited particle. The tape or slide carrying the particles may be passed through a staining bath 56. The tape after staining is scanned by close circuit television scanning device 40 which measures the distances between successive particles, the speed of relative movement of microscope and tape being the same as that between scanning device and tape, so that the measured physical characteristic for each particle can be correlated with its physical position on the slide using a computer (36). In the embodiment of Fig. 1 (not shown), the slide (18) is scanned by an optical microscope. Movement of the slide under the microscope can cease to allow e.g. observation of individual particles provided that the proper rate of movement is maintained between particles. In the embodiment of Fig. 3 (not shown), a slide (18) may be placed on a carriage movable along two axes under manual control, the position of each particle in the track being characterized by two scale readings.