METHOD OF BRINGING KNIFE CLOSE TO SPECIMEN IN MICROTOMB

摘要

A method for adjusting the position of the transparent triangular knife of a microtome with respect to a sample to be sectioned, consists of projecting a beam of light into the interior of the knife from below whereby an area of the sample above the knife edge will be illuminated by refraction of the projected beam from the upper sloping surface of the knife, the darkened area of the sample between the knife edge and the illuminated area constituting a measurement of the horizontal distance between the sample and the knife.