PRODUCTION OF LEPIDOPTERAN INSECT CELL LINES AND VIRUSES THEREIN

摘要

1441094 Insect cell line SHELL INTERNATIONALE RESEARCH MAATSCHAPPIJ BV 6 Nov 1973 [8 Nov 1972] 51555/72 Heading C6F [Also in Division A5] Lepidopteran insect cells are aerobically cultured in an insect haemolymph-free medium comprising assimilable carbon, mammalian serum, essential inorganic salts, vitamins and amino acids, at least part of the amino acid requirement being provided by a protein hydrolysate. The cells may be of Trichoplusia ni. The cells may be infected with an insect (NPN) virus, e.g. Autographa californica, before being cultured, the resulting viruses being harvested e.g. for inclusion with a carrier and a surface active agent in an insecticidal composition.