Analysis and Modeling of the Metabolism of Lepidopteran Cell Lines

摘要

The network metabolic model of High-FiveTM insect cells based on the consumption / production of metabolites and the protein, DNA/RNA, lipid and carbohydrates content of the cells has made it possible to study the internal fluxes of these cells' metabolism. In the last two decades, the Insect Cell - Baculoviras Expression Vector System (BEVS) has become popular for producing most recombinant (glyco-)proteins. The simplicity of insect cell cultivation in serum-free media and the easy construction of recombinant baculovirus vectors have made the BEVS quite an effective transient expression system. Among insect cell lines, High-Five and Sf-9 cells have great potential as technology platforms.