Fructose by glucose isomerisation - using controlled beds of arthrobacter genus cells contg heat stable glucose isomerase for higher purer yields

摘要

A 5-80 wt.% glucose soln. of viscosity 0.5-100.0 cps. and pH 6-9, pref. obtd. by enzymetric conversion of and liquefied starch, is heated to 20-80 degrees C, and passed through a bed (depth: dia. is 2:1 pref. 0.01:1 is 0.05:1) of cells of Arthrobacter sp. NRLL B-3726, NRRL B-3728 or ATCC 21748, that contains 3 IGIU)cm3 bed, the cells having a stability value of 300, pref. 400, hours and an extractability coefficient of 20%. The resulting fracture contg. soln., used to produce a sweeting agent has a colour value that has been increased by 2 colour units, and is purer and produced more economically than process using alkaline isomerization or glucose isomerase from Pseudomanas, Streptomyces, Bacillus, Acetobacter and Lactobacillus geni, by extn. or fixing.