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Enzymatic reagent and method for increasing the sensitivity of enzymatic substrate analyses using oxygen rate analyzers
Enzymatic reagent and method for increasing the sensitivity of enzymatic substrate analyses using oxygen rate analyzers
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机译:酶试剂和使用氧气速率分析仪提高酶底物分析灵敏度的方法
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摘要
A novel enzymatic reagent and method is provided for increasing the sensitivity of enzymatic substrate analyses using oxygen rate analyzers. The reagent is a buffered solution containing an enzyme which reacts specifically with oxygen and with the substrate being measured. The reagent also contains a salt or a combination of salts in quantities sufficient to reduce the solubility of oxygen in the reagent by at least thirty percent when compared to the solubility of oxygen in water. Upon being mixed with a specimen containing the specific enzyme substrate being measured, the enzyme catalyzes the reaction of the substrate and the oxygen. The rate of reaction of oxygen is measured, and the amount of total substrate is determined by means which translate the rate of oxygen consumption into the concentration of total substrate present in the specimen.
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