Composition for dyeing process for dyeing bacteria, bacteria, process for detecting bacteria in fluids, method for determining the susceptibility of bacteria to antimicrobials.And the process for deferenciar bacteriasgram negative Gram positive bacteria

摘要

STAINING AND ANALYSIS OF BACTERIAAbstract of the DisclosureA bacterial staining composition and methods ofanalysis of both gram-negative and gram-positive bacteriaare disclosed. The composition comprises a chelating agentand a basic dye, both of which are operative at a pH aboveabout 7Ø Bacterial staining may be effected by contactingeither concentrated or fluidly suspended bacteria with thecomposition at a neutral or basic pH. Bacteria stained withthe composition and concentrated by filtration, centrifugationor the like are readily visible and their presence in a specimenmay, thus, be rapidly detected. The gradations of color of thestained, concentrated bacteria correspond to the number ofbacteria and semi-quantitative analysis of the bacteria maybe effected by comparison with a standard. Differentiation ofgram-negative and gram-positive bacteria may be accomplished bytreating the stained bacteria with an organic acid wash havinga pH of about 2.5 to 2.6. Such a wash completely decolorizesonly stained gram-positive bacteria. Finally, a method fordetermining bacterial susceptibility to antimicrobial agentsis provided which comprises incubating bacteria with an anti-microbial agent, staining the bacteria and thereafter comparingthe color gradation of the stained, concentrated bacteria witha control or standard.