Isolation prod. from Phytolacca fruits - has anti-hepato-toxic properties and protects the liver

摘要

The novel isolation product (Ie from the fruits of Phytolacca species, esp. Phytolacca americana L., is characterised by the following: (i) m.pt. 246-7 degrees C (dec); (ii) Empirical formula C18H16O6, mol.wt. 328; (iii) Elemental analysis C:64.98%, H:4.98%, O:28.3%; (iv) Rf-value 0.17 or thin layer chromatography using silica gel -60-prepared plates (Merch) using CHCl3/acetone/HCOOH 9/2/1 (V-/V/V) as fluid; (v) sparingly soluble in water, CHCl3, benzene, petroleum ether, but soluble in DMSO, DMF, THF, dioxan and acetone; (vi) Optical degree of rotation alpha D17 of +23.7 degrees; and (vii) IR-absorption at 3200 cm-1 (-OH), 1650 cm-1 (alpha, beta-unsatd. C=O), 1610 cm-1 (double bond C=C double bond) and 1580, 1510 and 1450 cm-1 (aromatic systems). (I) has antihepatotoxic properties, producing a strong protective and stabilising effect on the cellular and intracellular biomembranes, esp. on the liver cells, thus protecting the liver. Suitable daily doses are 200-450 mg for humans. LD50 (i.v. mice) was 800-820 mg/kg.