use of certain strains of microorganisms for the production of cole sterinossidase that oxidizes the cole sterina with 02 to colestenone more h2 o2

摘要

Cholesterol oxidase which oxidizes cholesterol with oxygen to give cholestenone and hydrogen peroxide, is prepared by culturing Nocardia erthropolis ATCC 17895, Nocardia erthropolis ATCC 4277, Nocardia formica ATCC 14811 or Proactinomyce erythropolis NCIB 9158 first on a peptone-containing mineral salt medium and thereafter on at least one sterol compound as the sole source of carbon, and then isolating the cholesterol oxidase formed from the cultured micro-organism, wherein said sterol compound is of the formula IMAGE (I) in which R1 and R2 are hydrogen or together represent a double bond, R3 and R4 are hydrogen or together represent a double bond, and R5 is hydrogen or alkyl containing up to 3 carbon atoms.