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Method for the electrophoretic separation of proteins, a buffer solution and buffer compositions therefor
Method for the electrophoretic separation of proteins, a buffer solution and buffer compositions therefor
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机译:电泳分离蛋白质的方法,缓冲溶液及其缓冲组合物
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摘要
A buffer composition for use in the electrophoretic separation of proteins into fractions, the buffer composition consisting essentially of Tris; an acid having the formula Ri-CO-NH -R2 -COOH where R1 is NH2 or an alkyl or aryl group, preferably NH2, C6H5 or C6H4NH2 and where R2 is an alkyl group, preferably CH2, CH2-CH2 or CH(CH3); and a water soluble salt of the acid; the acid and salt being present in amounts and in a ratio to maintain the pH of an aqueous solution of the composition at from 8.2. to 9.0. The preferred acid is hippuric acid.;This application is a continuation-in-part of application Serial No. 92,250 filed 11-7-79 entitled "Buffer Composition and Method for the Electrophoretic Separation of Proteins", the entire disclosure of which is incorporated herein by reference.
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