Rapid determination of dissolved aflatoxins is effected by chromatography on a mini chromatographic column and comparison of the colour or fluorescence intensity of the resulting aflatoxin zones or bands with those of chromatogram zones or bands of a standard substance in known, graduated concns. in a similarly fitted control column, the colour or fluorescence intensity of the standard substance corresponding substantially to those of the aflatoxins to be determined. The mini chromatographic column is filled, in order (bottom upwards) with a layer of glass wool, granulated sodium sulphate, magnesium silicate gel ("Florisil"(RTM)), silica gel, neutral alumina, granulated sodium sulphate and a sealing layer of glass wool and is activated by heating 2 hrs. at 110 deg. A measured amt. of the solution mixture to be examined for aflatoxins is applied to the column, then the column is developed by descending chromatography using a suitable solvent mixture. Used for determination of aflatoxins, esp, in plant and animal extracts, e.g. to determine aflatoxins B1,B2,G1 and G2 in foodstuffs. Simple and rapid procedure which, because descending chromatography is used, permits the amount of developing solvent to be accurately measured and therefore gives improved reproducibility.
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