首页>
外国专利>
DNA capable of replication and stable mitotic maintenance in a host eukaryote, method of preparation thereof and eukaryotic cell containing same
DNA capable of replication and stable mitotic maintenance in a host eukaryote, method of preparation thereof and eukaryotic cell containing same
展开▼
机译:能够在宿主真核生物中复制和稳定有丝分裂维持的DNA,其制备方法和含有该DNA的真核细胞
展开▼
页面导航
摘要
著录项
相似文献
摘要
Functioning eukaryotic minichromosomes containing a centromere, at least one replicating site, and at least one functioning structural gene. Centromeres are obtained from a host chromosome and used as a genetic unit in combination with an autonomously replicating segment for high frequency stable transformation of host and other eukaryotic cells. The transformed eukaryotic cells can be cultivated through repeated passages without significant loss of the minichromosome and continued expression of the structural genes present in the minichromosome.
展开▼