TISSUE CULTURE OF PLANT BELONGING TO BORAGINACEAE FAMILY

摘要

PURPOSE:To produce a naphthoquinone compound and other useful components, efficiently, in bulk, by the tissue culture of a plant belonging to Boraginaceae family in a liquid medium containing ammonium ion as a nitrogen source and an organic acid at a specific ratio. CONSTITUTION:Tissue culture of a plant belonging to Boraginaceae family, e.g. Lipthospermum erythrorhizon Sieb. et Zucc. is carried out in a liquid medium obtained by adjusting the amount of ammonium ion in the medium for the conventional plant tissue culture to 0.1-80mol%, preferably 5-50mol% of the nitrogen source, and adding 1/20-5mol, preferably 1/5-2mol of one or more organic acid components selected from succinic acid, fumaric acid and malic acid per 1mol of the ammonium ion.