CALLUS CULTURE OF PLANT BELONGING TO BORAGINACEAE FAMILY

摘要

PURPOSE:To prepare naphthoquinone pigment and other useful materials, in large quantities, efficiently, by the callus culture of a plant belonging to Boraginaceae family in a liquid medium having controlled ratio of ammonium ion in the nitrogen source. CONSTITUTION:The callus culture of a plant belonging to Boraginaceae family, e.g. Lithospermum erythrorhizon Sieb. et Zucc, is carried out in a liquid medium such as Linsmeier-Skoog medium, etc., which contains a nitrogen source containing =10mol%, pref. = about 5mol% of ammonium ions, or esp. a nitrogen source essentially free from ammonium ion.